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Protein samples were dissolved in 62.5 mM Tris-Cl (pH 6.8) containing 2% (w/v) SDS and 15% (v/v) glycerol, separated by 10–20% sodium dodecyl surface-polyacrylamide gel electrophoresis, and blotted onto polyvinylidine difluoride membranes (Immobilon P Glutathione with Tretinoin & Azelaic Acid for Dark Spots | TikTok Variants:Bundle Flavor_Voodoo https://nordscipeptides.com/cdn/shop/files/hGH_Fragment_176-191_6mg.png?v=1767943811&width=1200

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