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The quantification of the DNA methylation of Nrf2 (Figure 4A) in a CpGs-rich amplicon revealed no difference between the ON and PN groups ( p = 0.57), while GSSG supplementation in PN attenuates DNA methylation levels when compared to ON and PN groups ( p = 0.03)
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Frozen sections were immersed in SDH action solution (R23066, SAINT-BIO, CHN) for 15 min and then rinsed, fixed, hydrated with ethanol, and then the sections were sealed with neutral adhesive, and images of the corresponding pathological changes were obtained using a microscope (DM2000, Leica, GER)
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