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Co-cultures were prepared with 3 × 10 4 target cells and varying effector-to-target (E: T) ratios (e.g., E:T of 5 with 1.5 × 10 5 effector cells) in 300 μL of 4T1-Vector CM or 4T1- Ldhb CM along with rIL-2 (1,000 U/mL)

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