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The following day, cells were fixed and incubated with an anti-BrdU antibody for 1 h at RT, followed by a 30-min incubation with horseradish peroxidase (HRP)-conjugated goat anti-mouse Immunoglobulin G (IgG) secondary antibody

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Plotting the 47 targeted plasma metabolites illustrates the shared and distinct metabolic signatures induced by CR and DL diets relative to AL-fed females (Fig