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Bacteria were washed twice with PBS before adding 50 μl bacterial suspensions and 50 μl of peptide (final 25 μM for 22 , 1 μM for 23 and 1 μM for 24 ) to a 96-well plate for flow cytometry (OD 595 of 1) and an 18-well IBIDI chamber pre-coated with poly-d-Lysine for imaging experiments (OD 595 of 0.25)

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The group prioritized the isolation of only the most statistically significant features, which resulted in a group of 37 compounds of interest, of which 18 molecules were increased in subjects and 19 decreased