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Cell death assessment in live ROs ROs were incubated with 10 µM PSVue-794 (Molecular Target Technologies, P-1001) or 4 µM ethidium homodimer (EtHD) (ThermoFisher Scientific, L3224) for 1 h and 45 min at 37 °C, washed three times with 1X-TES buffer and D-PBS, placed in clear media, and analyzed by 3D-ARQ