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Among them, the amino acid NH 3+ stretching vibration at 2,938 cm −1 , the sulfur hydrogen S-H stretching vibration at 2,524 cm −1 , the carboxylate COO stretching vibration at 1,712 cm −1 , the carbonyl C=O bond stretching (amide I) vibration at 1,660 cm −1 , and the C–N H–bond bending vibration (secondary amide amide II) at 1,395 cm −1 , the carboxylic acid root COO symmetric stretching vibration at 1,395 cm −1 , and the C–N stretching vibration caused by C–N stretching vibration at 1,248 cm −1