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Increase of fat oxidation and weight loss in obese mice caused by chronic treatment with human growth hormone or a modified C-terminal fragment | International Journal of Obesity

Mitochondrial injury from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) exposure produces OS

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Type:SubQ

MurE reaction: to 100 mM Tris buffer pH 7.9, 2.0 mM of one UDP-MurNAc-L-Ala-D-Glu derivative ( 1d – 3d , Supplementary Information), 4.0 mM meso-DAP (Sigma-Aldrich), 4.0 mM ATP, 2.0 mM MgCl 2 and 1.0 mM DTT was added 1.0 μg purified MurE enzyme per 100 μl reaction sample