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Tp53 −/− (KP) tumours by intratracheal delivery of lentiviruses expressing Cre recombinase and dual single guide RNAs (sgRNAs) 38 targeting Gpx4 (sg Gpx4 ) or non-targeting control sgRNAs (sg Neo ), enabling CRISPR–Cas9-mediated Gpx4 -knockout ( Gpx4 KO ) or wild-type control tumours, respectively

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Beyond cosmetic concerns, the potential anti-ageing effects of ergothioneine in the brain have also been of significant research interest, with in vivo data from animal models showing that ergothioneine protects neurons from damage by cisplatin (Reference Song, Chen and Liao72) , β-amyloid (Reference Yang, Lin and Wu73) , and age-related learning and memory deficits (Reference Song, Lin and Chen74)