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While GHK has been shown to inhibit the inflammatory response and epithelial-mesenchymal transition (EMT) via the TGF-β1/Smad 2/3 and IGF-1 pathways in bleomycin-induced fibrosis models [24], its broader anti-inflammatory effects in the context of pulmonary fibrosis pathogenesis may warrant further exploration
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0.04% p -nitrophenylphosphate (Sigma-Aldrich, CAS- 333338-18-4) were added and samples were incubated until the solution turned visibly yellow, before the reaction was stopped by adding 200 μL 1 M K 2 HPO 4
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