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These findings are also interesting from an IGF-1R targeting point of view because pharmacological modulations leading to an excess of MDM2 could be a manner to cause a selective IGF-1 inhibition and apoptosis in cancer cells
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Recovered proteins were separated by SDS-PAGE and electrophorectically transferred to nitrocellulose, after which they were blotted with IRDye 800CW streptavidin conjugate
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