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The cycling profile for real-time PCR (40 cycles) was as follows: 30 s at 95 °C for enzyme activation, 5 s at 95 °C for initial denaturation, 5 s at 65 °C for annealing/extension and a 5 s melt curve step at 65°C–95 °C
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Maintaining oxidative balance within these tissues supports normal digestive function
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