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Cell proliferation was evaluated using a Promega CellTiter 96™ AQueous One Solution Cell Proliferation Assay (MTS) as per the manufacturer’s instructions (Promega G3580)
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Post-mortem studies of brains from patients with HD have shown increased levels of DA and increased activity of tyrosine hydroxylase (TH), the rate-limiting enzyme in DA synthesis, in the striatum and SNc 3,4,5
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