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When present, classic findings include: Unilateral leg swelling (edema, increased limb circumference) Pain or tenderness along the course of the deep vein, often exacerbated by walking or dorsiflexion (Homans’ sign — low specificity, rarely documented) Erythema or skin warmth over the affected limb Skin discoloration — cyanosis (phlegmasia cerulea dolens in massive DVT) or pallor/blanching (phlegmasia alba dolens) Dilated superficial veins (collateral vessel prominence) Pitting edema distal to obstruction Upper extremity DVT may present with arm swelling, cyanosis, heaviness, or Paget-Schroetter syndrome (effort thrombosis in athletes/manual workers following strenuous use)

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Quantity:10 ml

For cycling assays, samples were diluted in 100 mM sodium phosphate buffer (pH 8) in a volume of 100 μl per well and added to white 96-well plates