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Next, the samples were concentrated in SpeedVac (Savant) under vacuum for 40 min, and the concentrated samples were re-constituted to the original volume by 10 μ l of Millipore water
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The purified TRF1 TRFH domain was then concentrated by centrifugation on a Vivaspin Turbo 15 centrifugal concentrator (10 kDa MWCO) to either ~ 3 mg/mL for NMR studies or ~ 30 mg/mL for crystallisation before flash-freezing and storage at −80 °C (Supplementary Fig
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