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The cell pellet was resuspended in chilled buffer (20 mM Tris pH 8.0, 150 mM NaCl) containing protease inhibitor cocktail and subjected to ultrasonication with a medium probe maintaining a temperature range of 6–12 °C (QSonica sonicator, 3 cycles of 90 s at power 7
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Cambridge D (1981): UK-14,304, a potent and selective alpha2-agonist for the characterisation of alpha-adrenoceptor subtypes
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