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The clinical study is registered in the registry www.clinicaltrials.gov (NCT03464500)and was conducted in accordance with the guidelines of the international council of harmonisation (ICH) for Good Clinical Practice (GCP) and the Declaration of Helsinki and follows the CONSORT reporting guidelines for randomized clinical trials Inclusion and exclusion criteria Study participants are aged ranged between 40 to 65 years and were above normal body weight (BMI between 25.0 and 34.9 kg/m 2 )

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Further worsening was caused by reperfusion for 60 min (i) or 30 min (ii/iii)