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His-tagged protein purification:- Recombinant proteins which have Histidine tags can be purified using immobilised metal-ion Chromatography (IMAC),and these tags can be placed at either the N-or C-terminus of the protein.Optimal binding and, therefore, purification efficiency is achieved when the His-tag is freely accessible to metal ion suppor

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[DOI] [PMC free article] [PubMed] [Google Scholar] 157.Desquesnes M., Gonzatti M., Sazmand A., Thévenon S., Bossard G., Boulangé A., Gimonneau G., Truc P., Herder S., Ravel S., et al