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Anesthesia typically develops within 20-30 minutes and may endure for 1-3 hours after removal
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Cell culture The human endometrial stromal cell line (HESC) (#T0533, ABM, Canada) was cultured in DMEM/F12 medium (#P04-41550, PAN-Biotech, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin
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